Toulligqc

Latest version: v2.7.1

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2.7.1

* Added Conda environment.yml.
* Fixed bugs:
* Corrected N50 and L50 computation (issue 28).
* Recognized the standard barcode arrangement (BC|RB|NB|BP) in the samplesheet (issue 27).
* Fixed barcoding for the Fastq_extractor (issue 30).
* Fixed time format for BAM and FASTQ extractors.

2.7

* Moved to Ubuntu 24.04.
* Added support for ONT samplesheet.
* ToulligQC now recognizes the barcode column name as 'barcode'.
* ToulligQC now supports irregular barcode naming; issue 22.
* Fixed bugs:
* Pandas inplace issue for FASTQ and onedsquare summary files.
* PlotlyJS inclusion for independent image exports.
* Corrected read/base count histogram.
* Handled empty lines in FASTQ file.
* Resolved compression issue in pod5 file.
* BAM extractor can now deal with any BAM, even if it has not been generated by Dorado.
* Handled barcode name when it's associated with kit_barcode.

2.6

* Support for the POD5 format.
* Handling of barcoding in BAM and FASTQ files.
* Support for Fast5 .tar files without compression.
* Fixed an issue with BAM tags extraction.
* Fixed the issue with the 'passes_filtering'-only sequencing summary.
* Fixed the bug related to numpy.bool with numpy version 1.24 or later.
* Improved compatibility with pandas 2.0.

2.5

* Fixed error when no failed reads were found (Issue 20).
* Fixed error when unclassified barcodes were missing.
* FASTQ and BAM files can now be used instead of the sequencing summary file.
* Added the ability to specify a barcode range (e.g., `--barcodes barcode01:barcode48`).

2.4

* In over time graphs (read length, PHRED score and translocation speed), now fill the gaps for the 75% and 25% to avoid filling glitch.
* Fix 2D density plot title style.
* Fix error when a summary file with barcode information was provided in addition of barcoding files (Issue 17), now the barcode files will be skipped with a warning message in case summary file with barcode information is provided.
* Add the selected speed and sample frequency of the run in the "Run statistics" table of the ToulligQC report

2.3

* Numpy 1.24 is now supported (thanks to Sean Black).
* Scatter plot of read length vs PHRED score has been replaced by a 2D density plot.
* Add bases per barcode distribution graphs.

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