Woltka

Changed
- Improved performance moderately ([192](https://github.com/qiyunzhu/woltka/pull/192)).
- Parameter `--chunk` is now the number of unique query sequences instead of the number of lines ([192](https://github.com/qiyunzhu/woltka/pull/192)).
- Updated GitHub Actions workflow.

Added
- Added parameter `-x|--exclude`, which will exclude query sequences that are mapped to given reference sequences (such as host genome, spike-in, vector, etc.) ([192](https://github.com/qiyunzhu/woltka/pull/192)).
- Added support for interleaved paired-end SAM files ([191](https://github.com/qiyunzhu/woltka/pull/191)).
- Added native support for PAF file format ([182](https://github.com/qiyunzhu/woltka/pull/182)).

Fixed
- Updated hyperlinks in documentation.

Changed
- Modified the command-line interface. Sub-commands under the `tools` menu were raised to the main menu. For example, `woltka tools collapse` now becomes `woltka collapse` ([176](https://github.com/qiyunzhu/woltka/pull/176)). The `tools` menus is still kept for backward compatibility ([#177](https://github.com/qiyunzhu/woltka/pull/177)).
- Improved efficiency of handling BIOM tables ([171](https://github.com/qiyunzhu/woltka/pull/171), [#175](https://github.com/qiyunzhu/woltka/pull/175)). This significantly reduced the memory consumption and runtime of the `collapse` command.
- The `--trim-sub` parameter now takes underscore (`_`) as the default separator, if not explicitly specified ([173](https://github.com/qiyunzhu/woltka/pull/173)).
- Updated installation protocol. Now Woltka can be Conda-installed without explicit pre-installation of biom-format ([174](https://github.com/qiyunzhu/woltka/pull/174)).

Added
- Upgraded the `collapse` command. Now it can collapse using internal hierarchies of feature IDs, such as genome-gene pairs, taxonomic lineages, and EC numbers. This upgrade enables stratified taxonomic/functional analysis of coord-matching ORF tables, without explicitly stratifying them during the classification step (which otherwise is time and space-consuming). The output should be identical to the that of the old method ([173](https://github.com/qiyunzhu/woltka/pull/173)).
- Added a feature to the `normalize` command, which can extract gene lengths from a gene coordinates file. This enables convenient length-based normalization of an existing gene (ORF) profile. For example, it can convert raw frequencies into RPK ([179](https://github.com/qiyunzhu/woltka/pull/179)).
- Extended description of stratification and collapsing protocols in the documentation.

Fixed
- Fixed a bug in parsing sample ID lists ([164](https://github.com/qiyunzhu/woltka/pull/164)).

Changed
- Used a single integer to store gene and read information in the ordinal mapper; use bitwise operations to parse the information. This significantly reduced memory consumption ([142](https://github.com/qiyunzhu/woltka/pull/142)).
- Improved alignment file processing efficiency ([153](https://github.com/qiyunzhu/woltka/pull/153)).
- Updated installation protocol. Currently a single `pip install` command should suffice ([145](https://github.com/qiyunzhu/woltka/pull/145)).
- Updated GitHub Actions test from Python 3.6 to Python 3.8. The program itself should continue to support Python 3.6+ ([158](https://github.com/qiyunzhu/woltka/pull/158)).

Added
- Added a naive algorithm for read-gene matching when the number of reads is small. This improves speed ([148](https://github.com/qiyunzhu/woltka/pull/148)).
- Added support for stdin as input. This lets the program take a variety of previously unsupported alignment formats ([155](https://github.com/qiyunzhu/woltka/pull/15)).
- Added or updated several pieces of documentation, such as a RefSeq tutorial ([157](https://github.com/qiyunzhu/woltka/pull/157)).
- Experimentally added a much accelerated ordinal mapper, powered by NumPy and Numba (branch `numba`) ([152](https://github.com/qiyunzhu/woltka/pull/152)).

Changed
- Migrated from Travis CI to GitHub Actions ([127](https://github.com/qiyunzhu/woltka/pull/127)).
- Made `--map-as-rank` default when only mapping file(s) are provided ([132](https://github.com/qiyunzhu/woltka/pull/132)).
- Renamed `--normalize|-z` as `--frac|-f` ([128](https://github.com/qiyunzhu/woltka/pull/128)).
- Modified core algorithm which slightly improved performance ([124](https://github.com/qiyunzhu/woltka/pull/124)).

Added
- Added `tool normalize` command, with multiple features ([124](https://github.com/qiyunzhu/woltka/pull/124)).
- Added the feature to collapse a stratified table ([126](https://github.com/qiyunzhu/woltka/pull/126)).
- Created an WoL FTP server, and added link to it ([118](https://github.com/qiyunzhu/woltka/pull/118)).
- Added an WoL standard operating procedure (`wolsop.sh`) and documentation ([116](https://github.com/qiyunzhu/woltka/pull/116)).
- Added first [citation](https://www.biorxiv.org/content/10.1101/2021.04.04.438427v1.abstract) of Woltka ([#111](https://github.com/qiyunzhu/woltka/pull/111)).
- Added protocols for Bowtie2 / SHOGUN and Fastp ([121](https://github.com/qiyunzhu/woltka/pull/121)).
- Added discussion about mapping uniqueness ([131](https://github.com/qiyunzhu/woltka/pull/131)).

Fixed
- Fixed free-rank classification subject not found issue ([120](https://github.com/qiyunzhu/woltka/pull/120)).
- Corrected paths to example files and directories ([117](https://github.com/qiyunzhu/woltka/pull/117)).

Changed
- Updated Qiita documentation ([107](https://github.com/qiyunzhu/woltka/pull/107)).
- Renamed "gOTU" with "OGU" ([104](https://github.com/qiyunzhu/woltka/pull/104)).

Added
- Published at PyPI. Can be installed by `pip install woltka` ([108](https://github.com/qiyunzhu/woltka/pull/108)).
- Added instructions for using MetaCyc and KEGG ([99](https://github.com/qiyunzhu/woltka/pull/99), [#101](https://github.com/qiyunzhu/woltka/pull/101)).
- Added `tool collapse` command, which supports one-to-many classification ([99](https://github.com/qiyunzhu/woltka/pull/99)).

Fixed
- Fixed Handling of zero length alignment ([105](https://github.com/qiyunzhu/woltka/pull/105)).

Added
- First official release.