- Many bug fixes (detailed here ).
- Improvements to the code behind the input modules to address issues with color image stacks.
- Adding a caching layer to image file handling which should improve performance for image stacks.
- Improving the compositing of overlays on images.
We have fixed a bug in the “fill holes” algorithm which will likely result in slightly different (and we feel, better) segmentations for IdentifyPrimaryObjects and IdentifySecondaryObjects modules with the “fill holes” option enabled. This will not affect most assays, but you may want to confirm this and continue to use 2.1.0 if reproducibility is a concern.