Methtuple

Latest version: v1.8.0

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1.3.0

New or improved features

* Support reads containing indels (closes [25](https://github.com/PeteHaitch/comethylation/issues/25) and [#45](https://github.com/PeteHaitch/comethylation/issues/45)).
* Refined process for dealing with overlapping reads (closes [15](https://github.com/PeteHaitch/comethylation/issues/15)).
* Improved documentation (closes [82](https://github.com/PeteHaitch/comethylation/issues/82) and [#84](https://github.com/PeteHaitch/comethylation/issues/84)).
* Python 3.4 support.

Minor improvements

* Added NEWS file.

Bug fixes

* Provide default `--ir1p` and `--ir2p` (closes [79](https://github.com/PeteHaitch/comethylation/issues/79)).
* Switched to *nix style line endings (\n), rather than the Windows line endings (\r\n), for the .tsv file (closes [83](https://github.com/PeteHaitch/comethylation/issues/83)).
* Switched back to samtools instead of Picard in helper_scipts/run_comethylation.sh (closes [78](https://github.com/PeteHaitch/comethylation/issues/78)).
* Updated examples to use Bismark v0.12.5.

Internal

* Simplified creation of m-tuples.
* Added `get_positions` function to get the reference positions of all bases in a read (including `None` if no reference base, such as inserted bases or soft-clipped read-positions).
* Added `process_overlap` function to process overlapping paired-end reads using the given `--overlap-check`

:(

* Running time of `v1.3` is 2-3x slower than `v1.2`. Partly because reads with indels are no longer skipped and partly because of code simplifications. Should be able to improve performance now that some internals have been simplified.

1.2.0

New or improved features

* Python 3 support (not yet Python 3.4 compatible due to limitations of current version of Pysam).
* Include strand of m-tuple in output.

Minor improvements
* Updated examples to use Bismark v0.12.2 (closes [65](https://github.com/PeteHaitch/comethylation/issues/65)).
* Update `data/run_comethylation.sh` to use command line interface introduced in `v1.1`.
* Improved filtering of specific read-positions.

Bug fixes
* Fixed bug that meant the sum of the overlap scores was incorrect for paired-end reads aligned to the OT-strand.

1.1.0

New or improved features

* Complete re-design and simplification of command line interface (closes [74](https://github.com/PeteHaitch/comethylation/issues/74))
* Reduced memory usage (addresses [64](https://github.com/PeteHaitch/comethylation/issues/64)).
* Added support for gzip and bzip2 output files (closes [69](https://github.com/PeteHaitch/comethylation/issues/69)).
* Added parallelisation via GNU parallel in `helper_scripts/run_comethylation.sh`(closes [68](https://github.com/PeteHaitch/comethylation/issues/68)).
* Ignore specific read-positions rather than just start or end (closes [71](https://github.com/PeteHaitch/comethylation/issues/71))

Bug fixes
* `--methylationType CHG` requires `--strandSpecific` (closes [72](https://github.com/PeteHaitch/comethylation/issues/72)).

Internal

* Refactored `WithinFragmentComethylationMTuple` class as part of memory usage improvements.

1.0.0

Initial public release

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