- bugfix: cutting an amplicon object now preserves features - Changed requirement for NetworkX to 1.8.1
0.6.4
Changed
- The pcr function and Anneal class can now deal with primers with ambiguous codons like R = A or G. In the resulting PCR product, the ambiguous nucleotides are preserved in the tails i.e. the primer part not annealing. The annealing part will have the sequence corresponding to the template.
0.6.3
Changed
- Dseqrecord.add_feature can now take a string or some other sequence as input. The assembly primers function can now produce primers for a circular assembly.
0.6.2
Changed
- Dseqrecord gained three new methods:
- isorf() method returning True or False.
- List_features() method returns a list of all features as a formatted ASCII table.
- Extract_feature() extracts a feature in the form os a new Dseqrecord object.
- Changes to how the primer_design functions work, especially assembly primers.
0.6.1
Changed
- Fixed a bug in the Dseqrecord synced method and removed the utils synced function.