Scipion-em-xmipptomo

Latest version: v3.24.6.0

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3.1.1

- First released version of the plugin documented properly in this file. The plugin contains the following 23 protocols:

xmipptomo - align transformations ( XmippProtAlignTransform ):
Protocol to rotate a series of alignments to a common reference defined by a
Subtomogram Average

xmipptomo - apply alignment subtomo ( XmippProtApplyTransformSubtomo ):
Apply alignment matrix and produce a new setOfSubtomograms, with each subtomogram aligned to its reference.

xmipptomo - apply alignment tilt-series ( XmippProtApplyTransformationMatrixTS ):
Compute the interpolated tilt-series from its transform matrix.

xmipptomo - connected components to ROIs ( XmippProtCCroi ):
This protocol adjust a SetOfCoordinates (which usually will come from a
connected componnent) to a ROI (region of interest) previously defined

xmipptomo - cltomo ( XmippProtCLTomo ):
Averages a set of subtomograms taking into account the missing edge.

xmipptomo - connected components ( XmippProtConnectedComponents ):
This protocol takes a set of coordinates and identifies connected
components among the picked particles.

xmipptomo - crop tomograms ( XmippProtCropTomograms ):
Protocol to crop tomograms using xmipp_transform_window.
The protocol allows to change the size of a tomogram/s, by removing the
borders defined by the users

xmipptomo - Filter coordinates by map ( XmippProtFilterCoordinatesByMap ):
Filter coordinate by map both given a mask or a resolucion map from a tomogram

xmipptomo - half maps ( XmippProtHalfMapsSubtomo ):
Create half maps from a SetOfSubtomograms and its alignment

xmipptomo - local Resolution MonoTomo ( XmippProtMonoTomo ):
Given a tomogram the protocol assigns local resolutions to each voxel of the tomogram.
To do that, thje protocol makes use of two half tomograms, called odd and even.
These tomograms are reconstructed with the same alignment parameter but using the
half of the data. For instance, the odd/even-images of the tilt series, or much
better using the odd/even frames of the movies (recommended). The result is a
tomogram with the values of local resolution.

xmipptomo - phantom create subtomo ( XmippProtPhantomSubtomo ):
Create subtomogram phantoms

xmipptomo - phantom tomograms ( XmippProtPhantomTomo ):
Create phantom tomograms with phantom particles and its coordinates with the right Scipion transformation matrix

xmipptomo - resize tilt-series ( XmippProtResizeTiltSeries ):
Wrapper protocol to Xmipp image resize applied on tilt-series

xmipptomo - resize tomograms ( XmippProtResizeTomograms ):
Protocol to to resize tomograms using xmipp_image_resize.
The protocol allows to change the size of a tomogram/s by means
of different methods

xmipptomo - imagej roi ( XmippProtRoiIJ ):
Tomogram ROI selection in IJ

xmipptomo - score/filter coordinates ( XmippProtScoreCoordinates ):
Scoring and (optional) filtering of coordinates based on different scoring
functions (carbon distance, neighbour distance)

xmipptomo - subtomo alignment consensus ( XmippProtScoreTransform ):
Protocol to score a series of alignments stored in a SetOfSubtomograms by
quaternion distance analysis.

xmipp_alignmentDistance ranges from 0º to 180º. Therefore, a 0º distance is the best and means alignment is the same.
The lower the score the more similar is the alignment.

xmipptomo - split tilt-series ( XmippProtSplitTiltSeries ):
Wrapper protocol to Xmipp split Odd Even on tilt-series

xmipptomo - map back subtomos ( XmippProtSubtomoMapBack ):
This protocol takes a tomogram, a reference subtomogram and a metadata with geometrical parameters
(x,y,z) and places the reference subtomogram on the tomogram at the designated locations (map back).
It has different representation options.

xmipptomo - subtomo projection ( XmippProtSubtomoProject ):

Project a set of volumes or subtomograms to obtain their X, Y or Z projection of the desired range of slices.

xmipptomo - subtomo subtraction ( XmippProtSubtractionSubtomo ):
This protocol subtracts a subtomogram average to a SetOfSubtomograms, which are internally aligned and
numerically adjusted in order to obtain reliable results. The adjustment and subtraction is perfomed by
xmipp_volume_subtraction program. A mask can be provided if the user wants to perform the subtraction in a
determined region.

xmipptomo - tiltseries FlexAlign ( XmippProtTsFlexAlign ):

Simple protocol to average TiltSeries movies as basic
motion correction. It is used mainly for testing purposes.

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