Filtus

Latest version: v1.0.5

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14.02.2016

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BUG FIX:
* Minor bug fixes.

14.02.2014

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NEW FEATURES:
* New option "Write data to text file" in the QC plot dialog window. If this is checked, then after plotting the user is prompted for a file name, and the point coordinates are saved to this file. The usual meta information is included (if not unchecked in the "Preferences menu").
This file can be useful for identification of points in the plots when plotting many samples, which disables the legend.

OTHER:
* When loading VCF- or VCF-like files with several genotype columns, the sample names (e.g. in the "Loaded samples" window) are displayed as "filename_sample". For example, in "vcf_example.vcf" (included in the example files folder) the header of the first genotype column is "NA00001", resulting in the name "example_files\vcf_example.vcf_NA00001". If the genotype columns lack names, then "sample1", "sample2" a.s.o are inserted.

* More informative error messages in QC plots.

BUG FIXES:
* The "Gender estimation" plot (one of the QC plots) erroneously included variants in the pseudoautosomal regions PAR1 (60001-2699520) and PAR2 (154931044-155270560) on X. These are now removed before calculating heterozygosity on X.

13.05.2014

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NEW FEATURES:
* A user friendly database GUI replaces the old database functions. The four main functions are 1) New database creation, 2) Adding samples to a database, 3) Extracting subsets of a database, 4) Searching a database for specific variants.
Importantly, the database formats are slightly changed from the previous version. The simple format has 6 columns: CHROM, POS, OBS = number of samples with the variant, HET = heterozygous observations, HOM = homozygous observations, AFREQ = allele frequency = (HET + 2*HOM) / total number of samples
The extended format has the same 6 columns, succeeded by one column for each sample, indicating the alternative allele count (0, 1 or 2) for each variant.

* General column splits: In the Input settings dialog, one can now indicate up to 2 columns with associated separators. For example, if the AD field in a VCF file is split on "," (comma), it will be replaced by columns AD_1, AD_2, ... .

OTHER:
* The layout of the "Input settings dialog" is slightly changed, to reflect some necessary changes in the internal file reading process. The most significant change is the Genotype format choice. The "VCF format" should be checked if the file has a VCF-like genotype format (i.e. a format column followed by individual sample columns) even if the file itself is not a vcf file. Examples of this include typical output from the Annovar annotation software.

* Toggle between long and short display of loaded sample names.

* Removed the automatic naming in VCF-like files lacking sample names. Instead the filename itself is used as sample name, and an error is raised if the file appear to have more than one sample.

* GUI appearance: Replaced the word "file" by "sample" many places, e.g. "Loaded samples".

BUG FIXES:
* Gene files with non-Windows end-of-line characters caused crashes.

* The VCF preamble reader was too simplistic, hindering files from loading in some cases.

11.12.2015

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NEW FEATURES:
* Help pages for many features are now available by clicking on question marks within FILTUS.
* De novo dialog: Added a gender selector for the child, which is important for correct handling of X-linked variants.

OTHER:
* Renamed "example_files" folder to "testfiles"
* New "data" folder, containing genelengths and genetic map distances.
* Lots of minor changes in preparation for publication.

10.11.2014

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NEW FEATURES:
* Some improvements of the autozygosity mapping implementation (AutEx). The user dialog is simplified with two major differences: 1) Instead of giving explicitly the (Leutenegger) parameters "f" and "a" as input, the user now simply indicates if the parental relation is thought to be "Close", "1st-2nd cousins" or "Distant". In most practical cases the results are not sensitive to this input. 2) Instead of having the user specifying a recombination map file, the program automatically uses the file "DecodeMap_thin.txt" located in the "example_files" folder.

* Meta information is now included in output files when saving the detected autozygous regions ("Save main window content"). This file can then be used in the "Restrict to regions"-filter.

10.09.2014

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NEW FEATURES:
* 'NA' entries are now treated as missing during column filtering (and will therefore be kept if the 'kim' box is checked). This behaviour was already in place for 'greater than' and 'less than' filtering, where all non-numerical entries are treated as missing.

BUG FIXES:
* NB: A bug in 'Gene sharing family' sometimes gave wrong output if healthy controls were given.

* Wrong order of the 'ALT%' columns in the output of 'de novo' algorithm.

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