Polarimetry

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.8.0 provides more flexibility by allowing the import of ROIs from the ROI Manager of [ImageJ/Fiji](https://imagej.net/software/fiji/). The ROI Manager in PyPOLAR further displays ILow values that can now be changed. Bugs with respect to the saving of csv/excel files in the edge detection mode and to the renumbering of ROIs in the ROI manager were corrected.

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.8.0/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.8.0/PyPOLAR_installer.exe)

[Install from PyPI](https://github.com/cchandre/Polarimetry/wiki/Install-and-run-PyPOLAR-with-PyPI)

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.7.0 includes additional save output formats for more flexibility with data post-processing, notably the possibility to export the measured parameters per pixel in a `.csv` format as well as composite image files compatible with [ImageJ/Fiji](https://imagej.net/software/fiji/) . For SHG, in addition to the parameter S, the parameters S2 and S4 are also included.

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.7.0/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.7.0/PyPOLAR_installer.exe)

[Install from PyPI](https://github.com/cchandre/Polarimetry/wiki/Install-and-run-PyPOLAR-with-PyPI)

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.6.4 improves the registration for 4 POLAR with a more ergonomic display of the registration. Minor bugs have been corrected regarding the slicing upon registration. Some error messages are now displayed when the wrong calibration is selected. PyPOLAR 2.6.4 includes now a link to create and customize the colorbars used in the analysis. Additional bugs were corrected concerning, e.g., the computation of the dark value. The pickle option for saving results has been removed.

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.6.4/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.6.4/PyPOLAR_installer.exe)

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.6.2 improves the registration for 4 POLAR. A possibility to change the SIFT parameters (contrastThreshold, sigma) is introduced. Tuning these parameters allows for a better registration. The quality of the registration is measured by the Pearson and the Manders correlation coefficients. The values of these parameters are exported in the Excel file.
The registration is now decoupled from the choice of order for the polarization angles in 4 POLAR techniques.

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.6.2/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.6.2/PyPOLAR_installer.exe)

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.6.1 improves the rescaling of the window/app. It includes the possibility to create an ROI from the Crop Manager once coordinates of the zoom are entered. There is now the possibility to save figures and reopen them for later use (.pyfig extension to be selected in the Options tab, Save output). The 4POLAR 2D technique has been now implemented. In addition, it corrects a series of minor bugs (notably, in the edge detection tool).

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.6.1/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.6.1/PyPOLAR_installer.exe)

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.5 corrects a series of minor bugs. It improves the ROI Manager (with interactive modifications); it includes the possibility to measure orientations with respect to a reference angle.

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.5/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.5/PyPOLAR_installer.exe)