Polarimetry

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.4 features an edge detection module to compute the edges of the intensity image, and compute orientation angles with respect to these edges (contours).

[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.4/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.4/PyPOLAR_installer.exe)

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.3 features an ROI manager which allows the user to work flexibly and interactively with multiple ROIs. The user can assign to each ROI a name and a group, select/deselect ROIs to analyze, or delete specific or all ROIs. The list of ROIs can further be saved and reloaded at the user's convenience for later analysis.


[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.3/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.3/PyPOLAR_installer.exe)

**Polarimetry Analysis goes pythonic!**

PyPOLAR is a Python-based app for analyzing polarization-resolved microscopy data to measure molecular orientation and order in biological samples.

PyPOLAR 2.2 extends the polarimetry analysis to include one-photon fluorescence (1PF), CARS, SRS, SHG, two-photon fluorescence (2PF) and 4POLAR 3D fluorescence. Provides the possibility to change the experimental offset angle as well as to choose the sense of polarization used for the calibration. Includes new features: monitoring the intensity per pixel in the image, saving the polar stack as an animated GIF, measuring distances in image, saving in the excel file additional parameters used for the analysis (dark value, offset, polarization, binning), drawing freehand ROIs, possibility to crop figures, modify the density of sticks and adding axes to the figures post-processing. Increased flexibility by saving the analysis as a compressed pickle file (.pbz2): reloading this file allows the user to re-generate the graphs of interest. Images shown within the application (Intensity and Thresholding tabs) can now be saved in a variety of formats. "4POLAR 2D" and "Edge detection" are under construction.


[Download for macOS](https://github.com/cchandre/Polarimetry/releases/download/v2.2/PyPOLAR.dmg)

[Download for Windows](https://github.com/cchandre/Polarimetry/releases/download/v2.2/PyPOLAR_installer.exe)

extends the polarimetry analysis beyond one-photon fluorescence to include CARS, SRS, SHG and two-photon fluorescence. Includes new features (displaying and saving a polar stack, erasing ROIs, saving figures in png format, option to differentially change stick density in horizontal vs vertical pixels, possibility to change the experimental offset angle, possibility to change the min and max values for the color-coding of variables). Fixes minor bugs related to binning.

fixes bug related to the use of batch processing, includes link to present website for checking updates and an "end of analysis" alert message for batch analysis, and provides a choice of distortion-corrected disc cones to use for analysis depending on the wavelength and acquisition data

- [Download for macOS](https://drive.google.com/open?id=1Ixq264klI6qsP6NEQv5Xtkl2ljirL9yY)

- [Download for Windows](https://drive.google.com/open?id=1GDaixvs8-ptwTaejkHT8jQgPeBPDrxcT)