Sequana

Latest version: v0.18.0

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0.4.2

~~~~~~~~~~~~~~~~~~~~~~

* Updates:

* pipeline: variant calling cleanup and finalised
* pipeline: denovo updated (busco) and cleanup and finalised
* pipeline: pacbio_qc finalised
* pipeline: rnaseq: finalised
* module pacbio: speed up initialisation; add a random_selection method; add a summary method;

* NEWS:

* Sequanix: can now load cluster config
* new rules: busco, busco_analysis, canu
* new pipeline: pacbio_denovo
* multiqc modules integrated in sequana. See :ref:`developers` for details.
* module snaketools: new function get_pipeline_stats
* new gallery example with statistics about the pipelines

* CHANGES:

* remove random() function from FastQ (useless and will be put in new module
simulation)

0.4.1

~~~~~~~~~~~~~~~~~~

* Update of Variant calling and denovo pipelines with HTML report creation
* Fix 421 (check for dot command in sequanix)
* Fix 420 (sequanix browser on Mac)
* sequana_coverage 417 division by 0 fixed
* snpeff bugs for special genbank cases fixed

0.4

~~~~~~~~~~~~~~~~~~~~~

* Master release for sequanix

0.3

~~~~~~~~~~~~~~~~~~~~~~~~~~~~

* BUG FIXES:

- sequanix:
- rulegraph issue on SLURM system. Avoid the os.chdir
- fastq_samples/ fastq module: fix histogram_gc_content maximum range
- rulegraph rule: fix issue 405 (spaces in path to snakefile)
- genome coverage was buggy for multi chromosome and circular option on. Fixed
- adapters/expdesign modules: fixe the case of design files with same sample
name and same index but different lanes.
- sequana_coverage. Fix Issue 416 (float division by zero)

* CHANGES:

- sequanix:
- snakemake output is now cleared when pressing RUN
- quality_control pipeline: default to atropos instead of cutadapt for
adapter trimming. Kraken: remove classified reads and keep
unclassified. Unclassified reads are now compressed.
unclassified reads that are also compressed now.

* NEW:

- pacbio module: cleanup and add funcion to convert input BAM into Fasta
- sequence module: Repeats class added
- new Snakemake pipeline called qc_pacbio to perform quick QC and taxonomy analysis
for pacbio
- add ORD, CDS, GC SKEW in sequence module.


0.2. - March - April 2017
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


* NEWS:
- RNA-seq pipeline added (single-end only, paired-end upcoming)
including all indexes for RNA-seq
- Hierarchical kraken available
- add new standalone called **sequana_fox** to expose the pyqt5 browser.
- Sequanix first release
- final version of the variant calling, denovo, quality_control and rna-seq
pipelines.

* CHANGES:

- Sequanix/Sequana:
- config file can have the yml extension (in addition to yaml)
- dropdown widgets in the form based on the docstrings in the config file
- can import config to override default sequana config file
- subprocesses killed when the main pipeline is stopped

0.1.21

~~~~~~~~~~~~~~~~~~~~~~~~

* NEWS:

- add sequana_debug_level function at top level to switch verbosity of
informative messages (default is WARNING).
- add pacbio module 351
- quality control pipeline: atropos can be used in place of cutadapt 346

* CHANGES:

- Running Median is 10 times faster 345
- sequana_coverage: (1) --file1 alone was not working (2) automatically copy
cluster-config in working directory and update runme.sh accordingly 342
- sequana standalone:
- handles cluster_config Snakemake option
- add error message when adapter name is incorrect
- sequanix: the help dialog is now created inside designer and has a proper
scrollable browser dialog. cluster_config Snakemake option is also handle.
- Remove galleria JS lib and related files (htmltools)
- sequana_coverage: add --logging-level option

* BUG:

- Fix 352 : allow gc window size to be even (warning is shown and +1 to
window size)
- Fix 354: cutadapt report that was mixing up R1/R2 trimming in the images.
- --output-directory in sequana_coverage was failing
- in coverage, centralness was buggy (regression) and use number of ROIs
instead of the total base length 347
- Fix multi_report summary for single end case 349

0.1.20

~~~~~~~~~~~~~~~~~~~~~~~~

* CHANGES:

- remove pyquickhelper dependencies and add a simple rest2html function in
misc module.

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