Spladder

Latest version: v3.1.0

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3.0.0

This is a new major release, as it introduces quite many changes, some of which break compatibility with some of the output of 2.4.x. and earlier versions.

Changelog:
* addition of a label to the output showing whether an intron is annotated in the given annotation
* addition of a label to each event indicating whether it contains one or several annotated vs novel introns (carry through annotation flag from input to output (per isoform))
* the naming convention of count features has been changed to be more general, also generalising the order of exons
* the support for pyproc (that was partially able to submit compute jobs to HPC systems) has been removed, instead use of workflow managers like Snakemake or Nextflow is recommended
* instead of only looking at the RNA-Seq evidence, an event can also be validated using the given annotation information (via a new command line switch `--use-anno-support`)
* chunksize of chunked merge is now configurable
* coordinate string computation has been added to event object
* output of test mode has been made more verbose (including coordinates and exon
usage)
* event ID spelling has been made consistent between build and test modes
* the documentation has been extended

Bugfixes:
* fixed a bug in validation code for mutually exclusive exons
* handling of None-type strand values (147)

2.4.4

bugfix release - correcting use of cram input in sparse mode

2.4.3

Bugfix release
- move to numpy and remove deprecation warnings
- address 115 and 122
- address parsing problem when input bam files and have the form *bam.bam

2.4.2

This release has the following changes:

* improved handling of CRAM input files
* fixing a bug leading to truncated sample names in distributed quantification

2.4.1

This release adds experimental support for CRAM files and updates some dependencies.

2.4.0

This release has the following changes:
* event IDs in text output are now more consistent (e.g., what previously was exon_skip.1 in gff and exon_skip_1 in txt output, now is consistently exon_skip.1)
* a command for the external chunked merge of many splicing graphs has been introduced (allowing for better distribution of large cohorts)
* some typos were fixed

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