Cutadapt

-----------------

* :issue:`292`: Implement support for demultiplexing paired-end reads that use
:ref:`combinatorial indexing (“combinatorial demultiplexing”)
<combinatorial-demultiplexing>`.
* :pr:`384`: Speed up reading compressed files by requiring an xopen version
that uses an external pigz process even for *reading* compressed input files
(not only for writing).
* :issue:`381`: Fix ``--report=minimal`` not working.
* :issue:`380`: Add a ``--fasta`` option for forcing that FASTA is written
to standard output even when input is FASTQ. Previously, forcing
FASTA was only possible by providing an output file name.

-----------------

* :issue:`378`: The ``--pair-adapters`` option, added in version 2.1, was
not actually usable for demultiplexing.

---------------------

* :issue:`376`: Fix a crash when using anchored 5' adapters together with
``--no-indels`` and trying to trim an empty read.
* :issue:`369`: Fix a crash when attempting to trim an empty read using a ``-g``
adapter with wildcards.

-----------------

* :issue:`366`: Fix problems when combining ``--cores`` with
reading from standard input or writing to standard output.
* :issue:`347`: Support :ref:`“paired adapters” <paired-adapters>`. One use case is
demultiplexing Illumina *Unique Dual Indices* (UDI).

-----------------

This is a major new release with lots of bug fixes and new features, but
also some backwards-incompatible changes. These should hopefully
not affect too many users, but please make sure to review them and
possibly update your scripts!

Backwards-incompatible changes
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

* :issue:`329`: Linked adapters specified with ``-a ADAPTER1...ADAPTER2``
are no longer anchored by default. To get results consist with the old
behavior, use ``-a ^ADAPTER1...ADAPTER2`` instead.
* Support for colorspace data was removed. Thus, the following command-line
options can no longer be used: ``-c``, ``-d``, ``-t``, ``--strip-f3``,
``--maq``, ``--bwa``, ``--no-zero-cap``.
* “Legacy mode” has been removed. This mode was enabled under certain
conditions and would change the behavior such that the read-modifying options
such as ``-q`` would only apply to the forward/R1 reads. This was necessary
for compatibility with old Cutadapt versions, but became increasingly
confusing.
* :issue:`360`: Computation of the error rate of an adapter match no longer
counts the ``N`` wildcard bases. Previously, an adapter like ``N{18}CC``
(18 ``N`` wildcards followed by ``CC``) would effectively match
anywhere because the default error rate of 0.1 (10%) would allow for
two errors. The error rate of a match is now computed as
the number of non-``N`` bases in the matching part of the adapter
divided by the number of errors.
* This release of Cutadapt requires at least Python 3.4 to run. Python 2.7
is no longer supported.

Features
~~~~~~~~

* A progress indicator is printed while Cutadapt is working. If you redirect
standard error to a file, the indicator is disabled.
* Reading of FASTQ files has gotten faster due to a new parser. The FASTA
and FASTQ reading/writing functions are now available as part of the
`dnaio library <https://github.com/marcelm/dnaio/>`_. This is a separate
Python package that can be installed independently from Cutadapt.
There is one regression at the moment: FASTQ files that use a second
header (after the "+") will have that header removed in the output.
* Some other performance optimizations were made. Speedups of up to 15%
are possible.
* Demultiplexing has become a lot faster :ref:`under certain conditions <speed-up-demultiplexing>`.
* :issue:`335`: For linked adapters, it is now possible to
:ref:`specify which of the two adapters should be required <linked-override>`,
overriding the default.
* :issue:`166`: By specifying ``--action=lowercase``, it is now possible
to not trim adapters, but to instead convert the section of the read
that would have been trimmed to lowercase.

Bug fixes
~~~~~~~~~

* Removal of legacy mode fixes also :issue:`345`: ``--length`` would not enable
legacy mode.
* The switch to ``dnaio`` also fixed :issue:`275`: Input files with
non-standard names now no longer lead to a crash. Instead the format
is now recognized from the file content.
* Fix :issue:`354`: Sequences given using ``file:`` can now be unnamed.
* Fix :issue:`257` and :issue:`242`: When only R1 or only R2 adapters are given, the
``--pair-filter`` setting is now forced to ``both`` for the
``--discard-untrimmed`` (and ``--untrimmed-(paired-)output``) filters.
Otherwise, with the default ``--pair-filter=any``, all pairs would be
considered untrimmed because one of the reads in the pair is always
untrimmed.

Other
~~~~~

* :issue:`359`: The ``-f``/``--format`` option is now ignored and a warning
will be printed if it is used. The input file format is always
auto-detected.

------------------

Features
~~~~~~~~

* Close :issue:`327`: Maximum and minimum lengths can now be specified
separately for R1 and R2 with ``-m LENGTH1:LENGTH2``. One of the
lengths can be omitted, in which case only the length of the other
read is checked (as in ``-m 17:`` or ``-m :17``).
* Close :issue:`322`: Use ``-j 0`` to auto-detect how many cores to run on.
This should even work correctly on cluster systems when Cutadapt runs as
a batch job to which fewer cores than exist on the machine have been
assigned. Note that the number of threads used by ``pigz`` cannot be
controlled at the moment, see :issue:`290`.
* Close :issue:`225`: Allow setting the maximum error rate and minimum overlap
length per adapter. A new :ref:`syntax for adapter-specific
parameters <trimming-parameters>` was added for this. Example:
``-a "ADAPTER;min_overlap=5"``.
* Close :issue:`152`: Using the new syntax for adapter-specific parameters,
it is now possible to allow partial matches of a 3' adapter at the 5' end
(and partial matches of a 5' adapter at the 3' end) by specifying the
``anywhere`` parameter (as in ``-a "ADAPTER;anywhere"``).
* Allow ``--pair-filter=first`` in addition to ``both`` and ``any``. If
used, a read pair is discarded if the filtering criterion applies to R1;
and R2 is ignored.
* Close :issue:`112`: Implement a ``--report=minimal`` option for printing
a succinct two-line report in tab-separated value (tsv) format. Thanks
to :user:`jvolkening` for coming up with an initial patch!

Bug fixes
~~~~~~~~~

* Fix :issue:`128`: The “Reads written” figure in the report incorrectly
included both trimmed and untrimmed reads if ``--untrimmed-output`` was used.

Other
~~~~~

* The options ``--no-trim`` and ``--mask-adapter`` should now be written as
``--action=mask`` and ``--action=none``. The old options still work.
* This is the last release to support `colorspace data <https://cutadapt.readthedocs.io/en/v1.18/colorspace.html>`_
* This is the last release to support Python 2.